bsm-51339M [Primary Antibody]
MITF (M2A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MITF

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 4286

Swiss Prot: O75030

Source: This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lee C. J. et al. Melanogenesis regulatory activity of the ethyl acetate fraction from Arctium lappa L. leaf on α-MSH–induced B16/F10 melanoma cells. Industrial Crops and Products,2019 138, 111581. Read more>>
VALIDATION IMAGES

Lane 1: A431; Lane 2: PC-12; Lane 3: NIH/3T3 cell lysate (20µg per lane) probed with bsm-51339M MITF (M2A1) Monoclonal Antibody at 1:2000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.


Lane 1: A431 cell lysates probed with MITF (M2A1) Monoclona Antibody, Unconjugated (bsm-51339M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.