bsm-51345M [Primary Antibody]
FZR (1C7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: FZR

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 51343

Swiss Prot: Q9UM11

Source: This FZR antibody is generated from a mouse immunized with a recombinant protein between1-496 amino acids from human FZR.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 55


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T/17; Lane 2: Hela cell lysates probed with FZR (1C7) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.