bsm-51346M [Primary Antibody]
AKT2 (16C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: AKT2

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 208

Swiss Prot: P31751

Source: This AKT2 antibody is generated from a mouse immunized with a recombinant protein between 272-481amino acids from human AKT2.

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 56

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20µg per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.


Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). Nuclear counter stain is DAPI (blue).