bsm-51358M [Primary Antibody]
SRC (2C2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: SRC

Immunogen Range: 1-350/536


Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 6714

Swiss Prot: P12931

Source: This SRC antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-350 amino acids from human SRC.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 60


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Jurkat; Lane 2: A431 cell lysate at 20 µg per lane, probed with bsm-51358M SRC (2C2) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.


Lane 1: A549 cell lysates probed with SRC (2C2) Monoclonal Antibody, Unconjugated (bsm-51358M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.