bsm-51363M [Primary Antibody]
RAB1B (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: RAB1B

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 81876

Swiss Prot: Q9H0U4

Source: This RAB1B antibody is generated from a mouse immunized with a recombinant protein between 1-201 amino acids from human RAB1B.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 22


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HeLa Cell lysates; Lane 2: A431 Cell lysates; Lane 3: U-20S Cell lysates; probed with RAB1B (3C3) Monoclonal Antibody, unconjugated (bsm-51363M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


A431 cells probed with using RAB1B (3C3) Monoclonal Antibody (green) unconjugated bsm-51363M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).