bsm-51365M [Primary Antibody]
RYK (1C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: RYK

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 6259

Swiss Prot: P34925

Source: This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 68


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human lung lysates; probed with RYK (1C1) Monoclonal Antibody, unconjugated (bsm-51365M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


A431 cells probed with RYK (1C1) Monoclonal Antibody (green) unconjugated bsm-51365M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).