bsm-51374M [Primary Antibody]
PGK1 (6C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: PGK1

Immunogen Range: 1-417/417


Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 5230

Swiss Prot: P00558

Source: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Kidney tissue lysates; Lane 5: Mouse Testis tissue lysates; Lane 6: Mouse Liver tissue lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human MOLT4 cell lysates probed with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.