bsm-51379M [Primary Antibody]
CGAS (7E3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: CGAS

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 115004

Swiss Prot: Q8N884

Source: This MB21D1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from human MB21D1.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. cGAMP can be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner (PubMed:26229115).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 59


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiaofei Wang. et al. Selenomethionine mitigate PM2.5-induced cellular senescence in the lung via attenuating inflammatory response mediated by cGAS/STING/NF-B pathway. ECOTOX ENVIRON SAFE. 2022 Dec;247:114266Read more>>
VALIDATION IMAGES

Lane 1: THP-1; Lane 2: MDA-MB-31 cell lysate at 20 µg per lane, probed with bsm-51379M MB21D1 (7E3) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.


25 ug total protein per lane of various lysates (see on figure) probed with CGAS monoclonal antibody, unconjugated (bsm-51379M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.