bsm-51395M [Primary Antibody]
PHB (12C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: PHB

Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 5245

Swiss Prot: P35232

Source: This antibody is generated from a mice immunized with a recombinant protein between 1-205 amino acids from human.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 30


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cell lysates; Lane 2: Rat Liver lysates; Lane 3: MCF-7 Cell lysates; Lane 4: NIH/3T3 Cell lysates; probed with PHB (12C6) Monoclonal Antibody, unconjugated (bsm-51395M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PHB (12C6) Monoclonal Antibody (bsm-51395M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.