bsm-51431M [Primary Antibody]
Histone H3 (9C2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Histone H3

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 8350

Swiss Prot: P68431

Source: This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: C6 Cell Lysates; Lane 2: HeLa Cell Lysates; probed with Histone H3 (809CT10.4.2) Monoclonal Antibody, unconjugated (bsm-51431M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.