Host: Mouse
Target Protein: PPP2R1B
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 5519
Swiss Prot: P30154
Source: This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Predicted Molecular Weight: 66
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: human brain; Lane 2: Jurkat; Lane 3: mouse brain; Lane 4: mouse lung; Lane 5: rat brain cell lysate at 20 µg per lane, probed with bsm-51443M PPP2R1B (1496CT356.164.25.226) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPP2R1B (6C6) Monoclonal Antibody (bsm-51443M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.