bsm-51459M

DATASHEET

Host: Mouse

Target Protein: NANOG

Clonality: Monoclonal

Entrez Gene: 79923

Swiss Prot: Q80Z64

Source: Recombinant human NANOG.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation.

Size: 100ul, 20uL

Concentration: Lot Dependent

Applications: WB(WB=1:500-2000)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)
IHC-P(IHC-P=1:400-800)

Predicted Molecular Weight: 36

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.