Host: Mouse
Target Protein: LC3B
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 81631
Swiss Prot: Q9GZQ8
Source: Recombinant protein of human LC3 (APG8).
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Applications: WB=1:500-1000，
Predicted Molecular Weight: 13
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 2Read more>>
- Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078Read more>>
Lane 1: Y79 (soluble fraction of cell extract) cell lysates; Lane 2: 293 transfected with human LC3 (whole cell extract) cell lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Brain tissue lysates; Lane 2: Rat Brain tissue lysates; Lane 3: Human Brain tissue lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.