bsm-51466M [Primary Antibody]
ALDH2 Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: ALDH2

Clonality: Monoclonal

Entrez Gene: 217

Swiss Prot: P05091

Source: Recombinant human ALDH2.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(WB=1:500)
IHC-P(IHC-P=1:400-800)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1:50)
ICC/IF(ICC/IF=1:50)

Predicted Molecular Weight: 54


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shen Xiaorong. et al. ALDH2 as an immunological and prognostic biomarker: Insights from pan-cancer analysis. MEDICINE. 2024 Apr;103(16):e3782Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with ALDH2 monoclonal antibody, unconjugated (bsm-51466M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.