Host: Mouse
Target Protein: ALDH3A2
Clonality: Monoclonal
Entrez Gene: 224
Swiss Prot: P51648
Source: Recombinant human ALDH3A2.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Applications: WB=1:500-1000，IHC-P=1:50-100，
Predicted Molecular Weight: 55
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ALDH3A2 Monoclonal Antibody, Unconjugated (bsm-51470M) at 1:200 overnight at 4°C, DAB staining.
Lane 1: WiDr cell lysates probed with ALDH3A2 Monoclonal Antibody, Unconjugated (bsm-51470M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates probed with ALDH3A2 Monoclonal Antibody, Unconjugated (bsm-51470M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.