bsm-51475M [Primary Antibody]
RPS6KB2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: RPS6KB2

Clonality: Monoclonal

Entrez Gene: 6199

Swiss Prot: Q9UBS0

Source: Recombinant human RPS6KB2 (His-tagged).

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphorylates specifically ribosomal protein S6.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000,IHC-P=1:200-500,IF=1:10-50,

Predicted Molecular Weight: 53


Predicted Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Jurkat cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse NIH/3T3 cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.