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Host: Mouse
Target Protein: GAD65
Immunogen Range: 101-200/585
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 2572
Swiss Prot: Q05329
Source: KLH conjugated synthetic peptide derived from human GAD2
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µl
Concentration: Lot Dependent
Applications:
WB(1:500-1000，)
IHC-F
IF
IHC-P
Predicted Molecular Weight: 65
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with GAD65 monoclonal antibody, unconjugated (bsm-51524M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-51524M) at 1:800 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.