bsm-51529M [Primary Antibody]
CYP3A4 Monoclonal Antibody
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Host: Mouse

Target Protein: CYP3A4

Immunogen Range: 201-300/503


Clonality: Monoclonal

Isotype: IgM

Entrez Gene: 1576

Swiss Prot: P08684

Source: KLH conjugated synthetic peptide derived from human CYP3A4

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-F(1:100-500)
IHC-P(1:100-500)
IF(1:100-500)

Predicted Molecular Weight: 55 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with CYP3A4 monoclonal antibody, unconjugated (bsm-51529M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.