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Host: Mouse
Target Protein: Erk2
Immunogen Range: 101-200/360
Clonality: Monoclonal
Isotype: IgG1 k
Entrez Gene: 5594
Swiss Prot: P28482
Source: KLH conjugated synthetic peptide derived from human Erk2
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 0.5ug/ul
Applications:
WB(WB(1:500-2000))
FCM(FCM(1μg/Test))
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Xenopus
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with ERK2 monoclonal antibody, unconjugated (bsm-51555M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK2 antibody (bsm-51555M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.