bsm-51558M [Primary Antibody]
UCHL1/PGP9.5 Monoclonal Antibody
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Host: Mouse

Target Protein: UCHL1/PGP9.5

Immunogen Range: 151-223/223


Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7345

Swiss Prot: P09936

Source: KLH conjugated synthetic peptide derived from human UCHL1/PGP9.5

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(WB=1:500-1000)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)
IHC-P(IHC-P=1:20-50)

Predicted Molecular Weight: 25


Cross Reactive Species: Xenopus laevis

Predicted Cross Reactive Species: Human
Mouse
Rat
Cow
Pig
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with UCHL1/PGP9.5 monoclonal antibody, unconjugated (bsm-51558M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.