bsm-51560M [Primary Antibody]
Nm23/NME1 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Nm23/NME1

Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 4830

Swiss Prot: P15531

Source: Recombinant human NME1 (His-tagged).

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-1000，IHC-P=1:25-50，

Predicted Molecular Weight: 17


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nm23/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nm23/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4°C, DAB staining.


Lane 1: MCF-7 cell lysates probed with Nm23/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.