bsm-51570M [Primary Antibody]
Prohibitin Monoclonal Antibody
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Host: Mouse

Target Protein: Prohibitin

Clonality: Monoclonal

Entrez Gene: 5245

Swiss Prot: P35232

Source: Recombinant human Prohibitin between 1-205 amino acids.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(WB=1:500-1000)
IHC-P(IHC-P=1:25-50)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)

Predicted Molecular Weight: 30


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:25 overnight at 4°C, DAB staining.


Lane 1: 293 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: NIH/3T3 cell lysates; Lane 5: Rat Liver tissue lysates probed with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.