DATASHEET
Host:
Mouse
Target Protein:
Prohibitin
Clonality:
Monoclonal
Entrez Gene:
5245
Swiss Prot:
P35232
Source:
Recombinant human Prohibitin between 1-205 amino acids.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:25 overnight at 4°C, DAB staining.
Lane 1: 293 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: NIH/3T3 cell lysates; Lane 5: Rat Liver tissue lysates probed with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.