Host: Mouse
Target Protein: PAH
Immunogen Range: 121-300/452
Clonality: Monoclonal
Entrez Gene: 5053
Swiss Prot: P00439
Source: KLH conjugated synthetic peptide derived from human PAH
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 0.5ug/ul
Applications: WB=1:500-1000，IHC-P=1:50-100，IHC-F=1:400-800，
Predicted Molecular Weight: 52
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:25 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:25 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:25 overnight at 4°C, DAB staining.
Lane 1: HepG2 cell lysates; Lane 2: Mouse liver tissue lysates; Lane 3: Rat liver tissue lysates probed with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.