bsm-51588M [Primary Antibody]
USP5 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: USP5

Clonality: Monoclonal

Isotype: IgG1 _

Entrez Gene: 8078

Swiss Prot: P45974

Source: Recombinant human USP5.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000，

Predicted Molecular Weight: 96


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: U251 cell lysates; Lane 2: PC-3 cell lysates; Lane 3: Mouse brain tissue lysates; Lane 4: Rat brain tissue lysates; Lane 5: Rat liver tissue lysates probed with USP5 Monoclonal Antibody, Unconjugated (bsm-51588M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Testis tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Rat Testis tissue lysates; Lane 7: Human U251 cell lysates; Lane 8: Human HeLa cell lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human MCF-7 cell lysates probed with USP5 Monoclonal Antibody, Unconjugated (bsm-51588M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.