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Host: Mouse
Target Protein: Calpain 2
Clonality: Monoclonal
Isotype: IgG2b
Entrez Gene: 824
Swiss Prot: P17655
Source: KLH conjugated synthetic peptide derived from human Calpain 2
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100uL
Concentration: 0.5ug/ul
Applications:
WB(WB=1:500-1000)
IHC-P(IHC-P=1:25)
IHC-F(IHC-F=1:25-100)
IF(IF=1:25)
Flow-Cyt(Flow-Cyt=1:100)
Predicted Molecular Weight: 78
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:25 overnight at 4°C, DAB staining.
Lane 1: A549 cell lysates; Lane 2: A431 cell lysates; Lane 3: SK-BR-3 cell lysates probed with Calpain 2 Monoclonal Antibody, Unconjugated (bsm-51592M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.