bsm-51607M [Primary Antibody]
XIAP Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: XIAP

Clonality: Monoclonal

Entrez Gene: 331

Swiss Prot: P98170

Source: Recombinant human XIAP.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-4000，IHC-P=1:25，IHC-F=1:25，

Predicted Molecular Weight: 55


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with XIAP Monoclonal Antibody, Unconjugated (bsm-51607M) at 1:25 overnight at 4°C, DAB staining.


Lane 1: Jurkat cell lysates; Lane 2: Raji cell lysates; Lane 3: Hela cell lysates probed with XIAP Monoclonal Antibody, Unconjugated (bsm-51607M) at 1:1000~4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Raji cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human A549 cell lysates probed with XIAP Monoclonal Antibody, Unconjugated (bsm-51607M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.