bsm-51636M [Primary Antibody]
USP2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: USP2

Clonality: Monoclonal

Isotype: IgG1 _

Entrez Gene: 9099

Swiss Prot: O75604

Source: Recombinant human USP2 between 1-258 amino acids.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-2000，

Predicted Molecular Weight: 68


Cross Reactive Species: Human
African Green Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MCF-7 cell lysates; Lane 2: PC-3 cell lysates; Lane 3: COS-7 cell lysates; Lane 4: Du145 cell lysates; Lane 5: SK-BR-3 cell lysates probed with USP2 Monoclonal Antibody, Unconjugated (bsm-51636M) at 1:2000 dilution and 4°C overnight incubation. Lysates/proteins at 20 µg per lane. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.