bsm-51636M [Primary Antibody]
USP2 Monoclonal Antibody
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Host: Mouse

Target Protein: USP2

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 9099

Swiss Prot: O75604

Source: Recombinant human USP2 between 1-258 amino acids.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Size: 100uL

Concentration: 0.5ug/ul

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 68


Cross Reactive Species: Human

Predicted Cross Reactive Species: Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with USP2 monoclonal antibody, unconjugated (bsm-51636M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.