bsm-51644M
[Primary Antibody]
EIF2S1 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: EIF2S1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1965
Swiss Prot: P05198
Source: Recombinant human EIF2A.
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at 2-8C for one year. Avoid repeated freeze/thaw cycles.
Background:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Size: 100ul
Concentration: 0.5ug/ul
Applications: WB=1:500-2000，
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH/3T3 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human K562 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.