bsm-51675M [Primary Antibody]
HIF1AN Monoclonal Antibody
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Host: Mouse

Target Protein: HIF1AN

Immunogen Range: 1-349/349


Clonality: Monoclonal

Entrez Gene: 55662

Swiss Prot: Q9NWT6

Source: KLH conjugated synthetic peptide derived from human HIF1AN

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminaltransactivation domain (CAD). Functions as an oxygen sensor and,under normoxic conditions, the hydroxylation prevents interactionof HIF-1 with transcriptional coactivators includingCbp/p300-interacting transactivator. Involved in transcriptionalrepression through interaction with HIF1A, VHL and histonedeacetylases. Hydroxylates specific Asn residues within ankyrinrepeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A andseveral other ARD-containing proteins. Also hydroxylates Asp andHis residues within ARDs of ANK1 and TNKS2, respectively.Negatively regulates NOTCH1 activity, accelerating myogenicdifferentiation. Positively regulates ASB4 activity, promotingvascular differentiation.

Size: 100uL

Concentration: 0.5ug/ul

Applications: WB(WB=1:500-2000)
ICC/IF(ICC/IF=1:20-50)

Predicted Molecular Weight: 38


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with HIF1AN monoclonal antibody, unconjugated (bsm-51675M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.