bsm-51677M [Primary Antibody]
Dnmt3a Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Dnmt3a

Clonality: Monoclonal

Entrez Gene: 1788

Swiss Prot: Q9Y6K1

Source: Recombinant human Dnmt3a.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:500-4000，

Predicted Molecular Weight: 100


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: T47D cell lysates; Lane 2: CCRF-CEM cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Hela cell lysates probed with Dnmt3a Monoclonal Antibody, Unconjugated (bsm-51677M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.