bsm-51705M [Primary Antibody]
UBE2C Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: UBE2C

Immunogen Range: 121-179/179


Clonality: Monoclonal

Entrez Gene: 11065

Swiss Prot: O00762

Source: KLH conjugated synthetic peptide derived from human UBE2C

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Size: 100ul

Concentration: 0.5ug/ul

Applications: WB=1:1000-4000，

Predicted Molecular Weight: 20


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T/17 cell lysates; Lane 2: A431 cell lysates probed with UBE2C Monoclonal Antibody, Unconjugated (bsm-51705M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.