bsm-51714M [Primary Antibody]
RIPK3 Monoclonal Antibody
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Host: Mouse

Target Protein: RIPK3

Clonality: Monoclonal

Entrez Gene: 11035

Swiss Prot: Q9Y572

Source: Recombinant human RIPK3.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Essential for programmed necrosis in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(WB=1:500-1000)

Predicted Molecular Weight: 57


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
  • Dan Zhao. et al. Copper exposure induces inflammation and PANoptosis through the TLR4/NF-B signaling pathway, leading to testicular damage and impaired spermatogenesis in Wilson disease. CHEM-BIOL INTERACT. 2024 Jun;396:11106Read more>>
  • Shuang Wang. et al. Dioscin exerts nephroprotective effects by attenuating oxidative stress and necroptosis-induced inflammation. INT IMMUNOPHARMACOL. 2024 Oct;140:112885Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with RIPK3 monoclonal antibody, unconjugated (bsm-51714M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.