DATASHEET
Host:
Mouse
Target Protein:
Cyclooxygenase 2
Clonality:
Monoclonal
Entrez Gene:
5743
Swiss Prot:
P35354
Source:
Recombinant human PTGS2.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
VALIDATION IMAGES
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human LOVO cell lysates probed with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-51741M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with PTGS2/Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-51741M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.