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Host: Rabbit
Target Protein: HP1 gamma
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 11335
Swiss Prot: Q13185
Source: Recombinant human HP1 gamma Protein
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
IF(1:100-500)
ICC/IF(1:50-200)
Predicted Molecular Weight: 21 kDa
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HP1 gamma) monoclonal Antibody, unconjugated (bsm-51782R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.