DATASHEET
Host:
Rabbit
Target Protein:
14-3-3 epsilon
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7531
Swiss Prot:
P62258
Source:
Recombinant human 14-3-3 epsilon protein, full length.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).
VALIDATION IMAGES
Lane 1: SH-SY-5Y; Lane 2: 293T lysate probed with 14-3-3 epsilon (2E2) Monoclonal Antibody (bsm-52003R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded mouse placenta section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 14-3-3 epsilon (2E2) Monoclonal Antibody (bsm-52003R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 14-3-3 epsilon (2E2) Monoclonal Antibody (bsm-52003R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.