DATASHEET
Host:
Rabbit
Target Protein:
14-3-3 sigma
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
2810
Swiss Prot:
P31947
Source:
Recombinant human 14-3-3 sigma protein, full length.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication
p53-regulated inhibitor of G2/M progression.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.