bsm-52009R [Primary Antibody]
ADAM17 (9E7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADAM17

Immunogen Range: 700-824/824


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6868

Swiss Prot: P78536

Source: Recombinant human ADAM17 protein, around 700-824aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8<sup> </sup> T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03Read more>>
VALIDATION IMAGES

Jurkat cell lysate probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4°C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.


IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Flow cytometric analysis of Hela cells with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.


Lane 1: Pancreas lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.