bsm-52012R [Primary Antibody]
Alas1 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Alas1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 211

Swiss Prot: P13196

Source: Recombinant human Alas1 protein, around C-terminal 200aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human JAR cell lysates; Lane 2: Human Huvec cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human HepG2 cell lysates ; Lane 5: Human MCF-7 cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human Raji cell lysates probed with Alas1 (1G11) Monoclonal Antibody, Unconjugated (bsm-52012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.