bsm-52017R
[Primary Antibody]
ARF6 (5H4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ARF6
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 382
Swiss Prot: P62330
Source: Recombinant human ARF6 protein, full length.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Human liver lysate probed with ARF6 (5H4) Monoclonal Antibody, Unconjugated (bsm-52017R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF-7 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ARF6 (5H4) Monoclonal Antibody (bsm-52017R) at 1:200 in blocking solution. Slides were incubated overnight at 4°C followed by FITC conjugated secondary antibody inclubation and DAPI staining of the nucleus.