bsm-52024R [Primary Antibody]
Bcl-XL (12C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Bcl-XL

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 598

Swiss Prot: Q07817

Source: Recombinant human Bcl-XL protein, around 1-100aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human A549 cell lysates probed with Bcl-XL Monoclonal Antibody, Unconjugated (bsm-5202M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.