bsm-52030R [Primary Antibody]
Cdk6 Recombinant Antibody
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Host: Rabbit

Target Protein: Cdk6

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1021

Swiss Prot: Q00534

Source: Recombinant human Cdk6 protein, full length.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:800 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.