DATASHEET
Host:
Rabbit
Target Protein:
Cyclin B1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
891
Swiss Prot:
P14635
Source:
Recombinant human Cyclin B1 protein, around N-terminal 150aa.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
PRODUCT SPECIFIC PUBLICATIONS
- Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃
overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Jurkat, 2: HeLa, 3: HT-29
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 55 kDa
Observed MW: 55 kDa