Recombinant human Cyclin B2 protein, around N-terminal 150aa.
Purified by Protein A.
Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse colon section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.