bsm-52045R [Primary Antibody]
Cyclin B2 (2F4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclin B2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9133

Swiss Prot: O95067

Source: Recombinant human Cyclin B2 protein, around N-terminal 150aa.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded mouse colon section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.