bsm-52048R
[Primary Antibody]
Cyclin E1 (4H7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cyclin E1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 898
Swiss Prot: P24864
Source: Synthetic peptide derive from human cyclin e1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Qiao Zhang. et al. G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression. Int J Med Sci. 2022; 19(1): 4764Read more>>
VALIDATION IMAGES
HepG2 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human laryngeal cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human laryngeal cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.