DATASHEET
Host:
Rabbit
Target Protein:
Cytokeratin 1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3848
Swiss Prot:
P04264
Source:
Recombinant human Cytokeratin 1 protein, around C-terminal 100aa.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
PRODUCT SPECIFIC PUBLICATIONS
- Xue-ting Hu. et al. The GRHL3-regulated long non-coding RNA lnc-DC modulates keratinocytes differentiation by interacting with IGF2BP2 and up-regulating ZNF750. J DERMATOL SCI. 2024 FebRead more>>
VALIDATION IMAGES
Lane 1: 293T Cell lysates; Lane 2: HeLa cell lysates; probed with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.