bsm-52056R [Primary Antibody]
Cytokeratin 16 (1A4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cytokeratin 16

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3868

Swiss Prot: P08779

Source: Recombinant human Cytokeratin 16 protein, around N-terminal 100aa.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human skin lysates; probed with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


A431 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded Human lung cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.