bsm-52060R
[Primary Antibody]
Cytokeratin 20 (1F10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cytokeratin 20
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 54474
Swiss Prot: P35900
Source: Recombinant human Cytokeratin 20 protein, around C-terminal 150aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Lovo Cell lysates; Lane 2: HCT116 Cell lysates; Lane 3: CRC lysates; probed with Cytokeratin 20 (1F10) Monoclonal Antibody (bsm-52060R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
CRC cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Cytokeratin 20 (1F10) Monoclonal Antibody (bsm-52060R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody (bsm-52060R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Human Lovo cell lysates; Lane 2:Human SW480 cell lysates; Lane 3: Human A549 cell lysates probed with Cytokeratin 20 (1F10) Monoclonal Polyclonal Antibody, Unconjugated (bsm-52060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody, Unconjugated (bsm-52060R) at 1:200 overnight at 4°C, DAB staining.