bsm-52061R [Primary Antibody]
Cytokeratin 2e (2F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cytokeratin 2e

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3849

Swiss Prot: P35908

Source: Recombinant human Cytokeratin 2e protein, around 350-500aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human skin lysates probed with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .


Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed and paraffin-embedded Human skin cancer tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human esophagus tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4°C, DAB staining.