bsm-52065R [Primary Antibody]
Cytokeratin 7 (6E6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cytokeratin 7

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3855

Swiss Prot: P08729

Source: Recombinant human Cytokeratin 7 protein, around C-terminal 150aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A549 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: SK-BR-3 Cell lysates; Lane 4: SW1990 cell lysates; probed with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:1000 overnight at 4ËšC. Followed by a conjugated secondary antibody.


A549 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.