bsm-52068R
[Primary Antibody]
ERK2 (3G1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ERK2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 55945595
Swiss Prot: P28482
Source: Recombinant human ERK2 protein, around C-terminal 150aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jae Hoon Kim. et al. Anti-PTK7 Monoclonal Antibodies Exhibit Anti-Tumor Activity at the Cellular Level and in Mouse Xenograft Models of Esophageal Squamous Cell Carcinoma. INT J MOL SCI. 2022 Jan;23(20):12195Read more>>
- Won-Sik Shin. et al. Knockdown of PTK7 Reduces the Oncogenic Potential of Breast Cancer Cells by Impeding Receptor Tyrosine Kinase Signaling. INT J MOL SCI. 2023 Jan;24(15):12173Read more>>
VALIDATION IMAGES
Lane 1: HeLa Cell lysates; Lane 2: PC-12 Cell lysates; Lane 3: CRC lysates; probed with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: K562 cell lysates; Lane 2: Siha cell lysates probed with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with ERK2 Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Large intestine lysates probed with ERK2 monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Large intestine lysates probed with ERK2monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Stomach lysates; Lane 2: Mouse Pancreas lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Stomach lysates probed with ERK2 monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:200 overnight at 4°C, DAB staining.